Steve Edwards, president of the Kyneton Aero Club, which manages Kyneton's airfield, said the local piloting community was close-knit and he did not believe the deceased were from the club.
“It's tragic,” Mr Edwards said.
“It is a tight community up here and I don't believe it is one of ours.”
A spokesman for Riddell Airfield, at Clarkfield, said it was not yet clear where the plane had taken off.